WB (1:2000), IHC (1:250), ICC/IF (1:1000), IP (5µg), optimal dilutions for assays should be determined by the user.
Application Notes:
Application Notes: 0.5 µg/ml was sufficient for detection of FKBP52 in 20 µg total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary
Immunohistochemistry analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
Western Blot analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1.5 µg/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Western Blot analysis of Human Cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1.5 µg/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
