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CaMKII Antibody, IgG1, Clone: [6G9], Unconjugated, Mouse, Monoclonal

CaMKII Antibody, IgG1, Clone: [6G9], Unconjugated, Mouse, Monoclonal

Catalog Number: BYT-ORB1822494

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Article Name:	CaMKII Antibody, IgG1, Clone: [6G9], Unconjugated, Mouse, Monoclonal
Biozol Catalog Number:	BYT-ORB1822494
Supplier Catalog Number:	orb1822494
Alternative Catalog Number:	BYT-ORB1822494-100
Manufacturer:	Biorbyt
Host:	Mouse
Category:	Antikörper
Application:	ELISA, ICC, IF, IHC, IP, RIA, WB
Species Reactivity:	Bovine, Human, Mouse, Rat
Immunogen:	Partially purified rat CaMKII
Conjugation:	Unconjugated
Alternative Names:	CaMKII, CaM kinase II, CaM KII, CaMK-II, CamK-II, CAMKII, CaMKii, CamKII, Camkii, camKII, camkII, camkii, CAMKIIalpha, CamKIIalpha, CAMKIId, CDPK1, dCAMKII, dCaMKII, DCK, CAMK2A, CAMK2B, CAMK2D, CAMK2G, CAMKA

Mouse Anti-Rat CaMKII alpha subunit Monoclonal IgG1

Clonality:	Monoclonal
Concentration:	1 mg/ml
Clone Designation:	[6G9]
Molecular Weight:	50-60kDa
Isotype:	IgG1
NCBI:	033922
UniProt:	P11798
Buffer:	PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
Target:	CaMKII

Application Dilute:	WB (1:10000), IHC (1:2000), ICC/IF (1:50), optimal dilutions for assays should be determined by the user.
Application Notes:	Application Notes: 0.1 µg/ml was sufficient for detection of CamKII in 20 µg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary

	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear Staining.
	Western Blot analysis of Rat brain membrane lysate showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000.
	Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.
	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Tissue: dissociated hippocampal neurons. Species: Mouse. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X.
	Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.

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