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HSP60 Antibody, IgG1, Clone: [LK1], Unconjugated, Mouse, Monoclonal

HSP60 Antibody, IgG1, Clone: [LK1], Unconjugated, Mouse, Monoclonal

Catalog Number: BYT-ORB1822504

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Article Name:	HSP60 Antibody, IgG1, Clone: [LK1], Unconjugated, Mouse, Monoclonal
Biozol Catalog Number:	BYT-ORB1822504
Supplier Catalog Number:	orb1822504
Alternative Catalog Number:	BYT-ORB1822504-200
Manufacturer:	Biorbyt
Host:	Mouse
Category:	Antikörper
Application:	ELISA, FC, IHC, IP, WB
Species Reactivity:	Bovine, Canine, Drosophila, Frog, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Sheep
Immunogen:	Recombinant human HSP60
Conjugation:	Unconjugated
Alternative Names:	HSPD1, HSP60, 60 kDa heat shock protein, mitochondrial, Chaperonin 60, CPN60, HuCHA60, Heat shock protein family D member 1, GroEL homolog, mitochondrial, GROEL, HLD4, HSP 60, HSP65, SPG 13

Mouse Anti-Human HSP60 Monoclonal IgG1

Clonality:	Monoclonal
Concentration:	1 mg/ml
Clone Designation:	[LK1]
Molecular Weight:	60kDa
Isotype:	IgG1
NCBI:	002147
UniProt:	P10809
Buffer:	PBS, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
Target:	HSP60

Application Dilute:	WB (1:20000), IHC (1:100), ICC/IF (1:100), IP (1:200), optimal dilutions for assays should be determined by the user.
Application Notes:	Application Notes: 0.05 µg/ml of SMC-110 was sufficient for detection of HSP60 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody

	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.
	Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.
	Western Blot analysis of Human Cell line lysates showing detection of Hsp60 protein using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1. Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK1, . Tissue: skin Fibroblasts. Species: Human. Fixation: Cold 100% methanol for 30 minutes at -20C. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: DAKO LSAB2 streptavidin-peroxidase system. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain. Left: control, Right: 24 hours after 7th passage of senescence.
	Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

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