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HSP70 Antibody, IgG1, Clone: [C92F3A-5], Unconjugated, Mouse, Monoclonal

HSP70 Antibody, IgG1, Clone: [C92F3A-5], Unconjugated, Mouse, Monoclonal

Catalog Number: BYT-ORB1822512

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Article Name:	HSP70 Antibody, IgG1, Clone: [C92F3A-5], Unconjugated, Mouse, Monoclonal
Biozol Catalog Number:	BYT-ORB1822512
Supplier Catalog Number:	orb1822512
Alternative Catalog Number:	BYT-ORB1822512-200
Manufacturer:	Biorbyt
Host:	Mouse
Category:	Antikörper
Application:	AM, Blocking, ELISA, FACS, FC, ICC, IEM, IF, IHC, WB
Species Reactivity:	Bovine, Canine, Drosophila, Fish, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Other, Porcine, Rabbit, Rat, Sheep
Immunogen:	Human HSP70
Conjugation:	Unconjugated
Alternative Names:	HSPA1A, HSPA1B, HSPA1, HSP70, HSP70-1, HSP70.1, HSP70-2, HSP72, HSP73, HSX70, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B

Mouse Anti-Human HSP70 Monoclonal IgG1

Clonality:	Monoclonal
Concentration:	1 mg/ml
Clone Designation:	[C92F3A-5]
Molecular Weight:	70kDa
Isotype:	IgG1
NCBI:	005336
UniProt:	P0DMV8
Buffer:	PBS pH 7.4, 50% glycerol, 0.1% sodium azide Storage buffer changes when conjugated
Target:	HSP70

Application Dilute:	WB (1:1000), IHC (1:10000), ICC/IF (1:1000), FACS (1:1000), optimal dilutions for assays should be determined by the user.
Application Notes:	Application Notes: 1 µg/ml of SMC-100 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody

	Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5. Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.
	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5. Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 16 hours at RT. Secondary Antibody: Biotin Goat Anti-Mouse.
	Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.
	Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92F3A-5. Tissue: Heat Shocked CD3+ CD8+ T cells. Species: Mouse. Primary Antibody: Mouse Anti-Hsp70: FITC Monoclonal Antibody at 1:1000.
	Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5. Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

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