0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
CELSR1
Application Dilute:
IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of rat brain tissue using CELSR1 antibody.
Immunohistochemical staining of human cervix cancer tissue using CELSR1 antibody.
Paraformaldehyde-fixed, paraffin embedded (human cervix cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CELSR1) Polyclonal Antibody, Unconjugated (orb182525) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CELSR1) Polyclonal Antibody, Unconjugated (orb182525) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. A, B: non-peptide blocking, a, b: peptide blocking negative control.
Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CELSR1 Polyclonal Antibody, Unconjugated (orb182525) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
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