Centrin 2 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB182543
Article Name: Centrin 2 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB182543
Supplier Catalog Number: orb182543
Alternative Catalog Number: BYT-ORB182543-50,BYT-ORB182543-100,BYT-ORB182543-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P
Species Reactivity: Human, Mouse
Immunogen: KLH conjugated synthetic peptide derived from human Centrin 2 (1-100/172aa)
Conjugation: Unconjugated
Alternative Names: CALT, CEN2, 1110034A02Rik, caltractin, CETN2_HUMAN, CETN2, Caltractin isoform 1, CETN2_MOUSE,
Centrin 2 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 20 kDa
UniProt: P41208
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: CETN2
Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Centrin 2) Polyclonal Antibody, Unconjugated (orb182543) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Centrin 2) Polyclonal Antibody, Unconjugated (orb182543) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Tissue/Cell: Human laryngeal tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Centrin 2 Polyclonal Antibody, Unconjugated (orb182543) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human laryngo carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Centrin-2 Polyclonal Antibody, Unconjugated (orb182543) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: Human lung cancer tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Centrin 2 Polyclonal Antibody, Unconjugated (orb182543) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.