A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the recombinant CD4 antibody.
Conjugation:
Unconjugated
Recognizes a protein of 55kDa, identified as CD4. It is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. The majority of peripheral T-cell lymphomas are derived from the T-helper/regulatory cell subset so that most mature T-cell neoplasms are CD4+/CD8-. Anti-CD4 is used in the immunohistochemical staining of lymphoproliferative disorders to evaluate tumors with CD4 aberrant expression.
1 mg/ml in 1X PBS, rAlbumin free, sodium azide free
Application Dilute:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT,Western blot: 2-4ug/ml
Application Notes:
Application Notes: Optimal dilution of the recombinant CD4 antibody should be determined by the researcher
IHC staining of FFPE human tonsil tissue with recombinant CD4 antibody (clone CD4/8203R) at 2 ug/ml. HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant CD4 antibody (clone CD4/8203R) at 2 ug/ml. HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder cancer pT24 with recombinant CD4 antibody (clone CD4/8203R) at 2 ug/ml. HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC testing of FFPE human brain tissue with recombinant CD4 antibody (clone CD4/8203R) at 2 ug/ml. HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD4 antibody (clone CD4/8203R) as confirmation of integrity and purity.
Western blot testing of human tonsil tissue lysate with recombinant CD4 antibody. Expected molecular weight: 50-55 kDa.
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