CRNKL1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB182753
Article Name: CRNKL1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB182753
Supplier Catalog Number: orb182753
Alternative Catalog Number: BYT-ORB182753-50,BYT-ORB182753-100,BYT-ORB182753-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human CRNKL1 (201-300/848aa)
Conjugation: Unconjugated
Alternative Names: CLF, CRN, Clf1, HCRN, MSTP021, SYF3, Q5JY65_HUMAN, CRNKL1, Crooked neck homolog, CRNL1_HUMAN, Crooked neck homolog (hCrn), CRNL1_RAT, Crooked neck protein,
CRNKL1 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 100 kDa
UniProt: Q5JY65
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: CRNKL1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of rat testis tissue using CRNKL1 antibody.
Immunohistochemical staining of mouse intestine tissue using CRNKL1 antibody.
Sample: Siha (Human) Cell Lysate at 30 ug, HepG2 (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, Primary: Anti-CRNKL1 (orb182753) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100 kD, Observed band size: 98 kD.
Tissue/Cell: mouse intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CRNKL1 Polyclonal Antibody, Unconjugated (orb182753) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CRNKL1 Polyclonal Antibody, Unconjugated (orb182753) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.