FLIP Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB182897
- Images (7)
| Article Name: | FLIP Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB182897 |
| Supplier Catalog Number: | orb182897 |
| Alternative Catalog Number: | BYT-ORB182897-50,BYT-ORB182897-100,BYT-ORB182897-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human CFLIP (1-100/480aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, cFLIP, 2310024N18Rik, A430105C05Rik, Gm9845, CFLAR_HUMAN, CFLAR, Caspase homolog (CASH), Caspase-eight-related protein (Casper), Caspase-like apoptosis regulatory protein (CLARP), Cellular FLICE-like inhibitory protein (c-FLIP), FADD-like antiapoptotic molecule 1 (FLAME-1), Inhibitor of FLICE (I-FLICE), MACH-related inducer of toxicity (MRIT), Usurpin, CFLAR_MOUSE, |
| FLIP Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 43 kDa |
| UniProt: | O15519 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | CFLAR |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CFLAR) Polyclonal Antibody, Unconjugated (orb182897) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat heart tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CFLAR) Polyclonal Antibody, Unconjugated (orb182897) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CFLAR) Polyclonal Antibody, Unconjugated (orb182897) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Human Panc-1 cell lysates, Lane 2: Human Huvec cell lysates, Lane 3: Human K562 cell lysates, Lane 4: Human HepG2 cell lysates, Primary: Anti-FLIP (orb182897) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kDa, Observed band size: 58 kDa. |
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Sample: Muscle (Mouse) Cell Lysate at 40 ug, Primary: Anti-CFLIP (orb182897) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 43 kD. |
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Sample: Skeletal muscle (Mouse) Cell Lysate at 40 ug, Primary: Anti-CFLIP (orb182897) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 43 kD. |
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Western blot:Muscle(Mouse) Cell,Primary:Anti-CFLIP at 1:300 dilution. |
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