DNAJC2 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB183162
Article Name: DNAJC2 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB183162
Supplier Catalog Number: orb183162
Alternative Catalog Number: BYT-ORB183162-50,BYT-ORB183162-100,BYT-ORB183162-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Rat
Immunogen: KLH conjugated synthetic peptide derived from human DNAJC2 (521-621/621aa)
Conjugation: Unconjugated
Alternative Names: MPHOSPH11, MPP11, ZRF1, ZUO1, MIDA1, Zrf2, DNJC2_BOVIN, DNAJC2, Zuotin-related factor 1, DNJC2_HUMAN, M-phase phosphoprotein 11, DNJC2_MOUSE, Mouse Id associate 1 (MIDA1), DNJC2_RAT, Gliosarcoma-related antigen MIDA1,
DNAJC2 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 72 kDa
UniProt: Q99543
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: DNAJC2
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of rat spleen tissue using DNAJC2 antibody.
25 ug total protein per lane of various lysates probed with DNAJC2 polyclonal antibody, unconjugated (orb183162) at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DNAJC2) Polyclonal Antibody, Unconjugated (orb183162) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DNAJC2) Polyclonal Antibody, Unconjugated (orb183162) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Tissue/Cell: rat spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-DNAJC2 Polyclonal Antibody, Unconjugated (orb183162) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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