DRD1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB183263
Article Name: DRD1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB183263
Supplier Catalog Number: orb183263
Alternative Catalog Number: BYT-ORB183263-50,BYT-ORB183263-100,BYT-ORB183263-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Rat
Immunogen: KLH conjugated synthetic peptide derived from human Dopamine Receptor D1 (11-100/446aa)
Conjugation: Unconjugated
Alternative Names: D1R, DADR, DRD1A, C030036C15Rik, Drd-1, Gpcr15, D1a, DRD1_HUMAN, DRD1, Dopamine D1 receptor, DRD1_MOUSE, DRD1_RAT,
DRD1 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 65-80 kDa
UniProt: P21728
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: DRD1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
Flow cytometric analysis of SH-SY5Y Cell using Dopamine Receptor D1 antibody.
Cell: SH-SY5Y, Concentration: 1:100, Host/Isotype: Rabbit/IgG, Flow cytometric analysis of Rabbit IgG isotype control (Cat: orb183263) on SH-SY5Y (green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody.
Sample: A431 Cell (Human) Lysate at 40 ug, HepG2 Cell (Human) Lysate at 40 ug, Primary: Anti-Dopamine Receptor D1 (orb183263) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50kD, Observed band size: 60kD.
Sample: A549 Cell (Human) Lysate at 40 ug, Primary: Anti-Dopamine Receptor D1 (orb183263) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50kD, Observed band size: 60kD.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Dopamine Receptor D1 Polyclonal Antibody, Unconjugated (orb183263) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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