Phospho-HSF1 (Ser303 + Ser307) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB184051
Article Name: Phospho-HSF1 (Ser303 + Ser307) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB184051
Supplier Catalog Number: orb184051
Alternative Catalog Number: BYT-ORB184051-50,BYT-ORB184051-100,BYT-ORB184051-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human HSF1 around the phosphorylation site of Ser303+Ser307 PP(p-S)PPQ(p-S)PR
Conjugation: Unconjugated
Alternative Names: HSF1 (p-S303/S307), p-HSF1, phospho-HSF1, HSTF1, HSTF, HSTF 1, Hsf1alpha, Hsf1beta, HSF1_HUMAN, HSF1, HSF 1, Heat shock transcription factor 1 (HSTF 1), HSF1_MOUSE,
Phospho-HSF1 (Ser303 + Ser307) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 57 kDa
UniProt: Q00613
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: HSF1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Application Notes: Modification: Phosphorylated
Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSF1 (Ser303 + Ser307)) Polyclonal Antibody, Unconjugated (orb184051) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (phospho-HSF1 (Ser303 + Ser307)) Polyclonal Antibody, Unconjugated (orb184051) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (phospho-HSF1 (Ser303 + Ser307)) Polyclonal Antibody, Unconjugated (orb184051) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (phospho-HSF1 (Ser303 + Ser307)) Polyclonal Antibody, Unconjugated (orb184051) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human MDA-MB-231 cell lysates, Primary: Anti-phospho-HSF1 (Ser303 + Ser307) (orb184051) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 78 kD.



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