Lin28 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB1842
Article Name: Lin28 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB1842
Supplier Catalog Number: orb1842
Alternative Catalog Number: BYT-ORB1842-50,BYT-ORB1842-100,BYT-ORB1842-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human Lin28 (75-180/209aa)
Conjugation: Unconjugated
Alternative Names: CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A, Gm10299, Tex17, RGD1566408, LN28A_HUMAN, LIN28A, Zinc finger CCHC domain-containing protein 1, LN28A_MOUSE, Testis-expressed protein 17,
Lin28 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 29 kDa
UniProt: Q9H9Z2
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: LIN28A
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200
Immunohistochemical staining of rat testis tissue using Lin28 antibody.
Immunohistochemical staining of mouse embryo tissue using Lin28 antibody.
Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Lin28) Polyclonal Antibody, Unconjugated (orb1842) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Rat Testis tissue lysates, Lane 3: Rat Heart tissue lysates, Primary: Anti-Lin28 (orb1842) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kDa, Observed band size: 28 kDa.
Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Lin28 Polyclonal Antibody, Unconjugated (orb1842) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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