HTRA4 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB184261
Article Name: HTRA4 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB184261
Supplier Catalog Number: orb184261
Alternative Catalog Number: BYT-ORB184261-50,BYT-ORB184261-100,BYT-ORB184261-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human HTRA4 (251-350/476aa)
Conjugation: Unconjugated
Alternative Names: B430206E18Rik, HTRA4_HUMAN, HTRA4, High-temperature requirement factor A4, 3.4.21.-, HTRA4_MOUSE,
HTRA4 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 49 kDa
UniProt: P83105
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: HTRA4
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of rat pancreas tissue using HTRA4 antibody (dilution at 1:500)
WB analysis of (human/cell line) DU145 lysatesprobed using HTRA4 antibody (dilution at 1:300)
Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (HTRA4) Polyclonal Antibody, Unconjugated (orb184261) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Protein: DU145 (human) lyates at 30 ug, Primary: Rabbit Anti-HTRA4 at 1:300, Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG (H+L) at 1: 10000, Predicted band size: 49 kD, Observed band size: 50 kD.
Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Primary: Anti-HTRA4 (orb184261) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kDa, Observed band size: 50 kDa.
Sample: PC-3 Cell (Human) Lysate at 30 ug, Primary: Anti-HTRA4 (orb184261) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.
Tissue/Cell: Rat pancreas tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-HTRA4 Polyclonal Antibody, Unconjugated (orb184261) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



* VAT and and shipping costs not included.
Errors and price changes excepted
Availability
ORDER BY EMAIL
Product Guarantee and Expert Support
BIOZOL