0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
MAPK8
Application Dilute:
IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (JNK1+JNK2) Polyclonal Antibody, Unconjugated (orb184468) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (JNK1+JNK2) Polyclonal Antibody, Unconjugated (orb184468) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (JNK1+JNK2) Polyclonal Antibody, Unconjugated (orb184468) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Western blot:Spinal cord (Rat),Medulla (Rat),Primary:Anti-JNK1+JNK2 at 1:1000 dilution.
Western blot:Spinal cord (Mouse),Medulla (Mouse),Primary:Anti-JNK1+JNK2 at 1:1000 dilution.
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