KMT4 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB184545
Article Name: KMT4 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB184545
Supplier Catalog Number: orb184545
Alternative Catalog Number: BYT-ORB184545-50,BYT-ORB184545-100,BYT-ORB184545-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human KMT4 (51-150/1739aa)
Conjugation: Unconjugated
Alternative Names: DOT1, KMT4, NDNS, DOT1L_HUMAN, DOT1L, DOT1-like protein, Histone H3-K79 methyltransferase (H3-K79-HMTase), Lysine N-methyltransferase 4, 2.1.1.360, KIAA1814,
KMT4 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 185 kDa
UniProt: Q8TEK3
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: DOT1L
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of human kidney tissue using KMT4 antibody.
Immunohistochemical staining of human kidney tissue using KMT4 antibody.
Sample: Liver (Mouse) Lysate at 40 ug, Liver (Rat) Lysate at 40 ug, Primary: Anti-KMT4 (orb184545) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 185 kD, Observed band size: 170 kD.
Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-KMT4 Polyclonal Antibody, Unconjugated (orb184545) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-KMT4 Polyclonal Antibody, Unconjugated (orb184545) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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