Phospho-LIM Kinase 1 (Thr508) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB184928
- Images (7)
| Article Name: | Phospho-LIM Kinase 1 (Thr508) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB184928 |
| Supplier Catalog Number: | orb184928 |
| Alternative Catalog Number: | BYT-ORB184928-50,BYT-ORB184928-100,BYT-ORB184928-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthesised phosphopeptide derived from human LIM Kinase 1 around the phosphorylation site of Thr508 RY(p-T)VV |
| Conjugation: | Unconjugated |
| Alternative Names: | LIMK1 (p-S293), p-LIMK1, phospho-LIMK1, LIMK, LIMK-1, KIZ-1, LIMK1_HUMAN, LIMK1, 2.7.11.1, LIMK1_MOUSE, LIMK1_RAT, |
| Phospho-LIM Kinase 1 (Thr508) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 73 kDa |
| UniProt: | P53667 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | LIMK1 |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Application Notes: | Modification: Phosphorylated |
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Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-LIM Kinase 1 (Thr508)) Polyclonal Antibody, Unconjugated (orb184928) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-LIM Kinase 1 (Thr508)) Polyclonal Antibody, Unconjugated (orb184928) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-LIM Kinase 1 (Thr508)) Polyclonal Antibody, Unconjugated (orb184928) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-LIM Kinase 1 (Thr508)) Polyclonal Antibody, Unconjugated (orb184928) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-LIM Kinase 1 (Thr508)) Polyclonal Antibody, Unconjugated (orb184928) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-LIM Kinase 1 (Thr508)) Polyclonal Antibody, Unconjugated (orb184928) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-LIM Kinase 1 (Thr508)) Polyclonal Antibody, Unconjugated (orb184928) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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