Phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB185140
- Images (7)
| Article Name: | Phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB185140 |
| Supplier Catalog Number: | orb185140 |
| Alternative Catalog Number: | BYT-ORB185140-50,BYT-ORB185140-100,BYT-ORB185140-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthesised phosphopeptide derived from human MST4/MST3/STK25 around the phosphorylation site of Thr178/Thr190/Thr174 RN(p-T)FV |
| Conjugation: | Unconjugated |
| Alternative Names: | MST4 & MST3 & STK25 (p-T178/T190/T174), p-MST4 & MST3 & STK25, phospho-MST4 & MST3 & STK25, SOK1, YSK1, MASK, MST4, HEL-S-95, MST3, MST3B, STE20, STK3, STK25_HUMAN, STK25, Ste20-like kinase, Sterile 20/oxidant stress-response kinase 1 (SOK-1 | Ste20/oxidant stress response kinase 1), 2.7.11.1, STK26_HUMAN, STK26, MST3 and SOK1-related kinase, Mammalian STE20-like protein kinase 4 (MST-4 | STE20-like kinase MST4), Serine/threonine-protein kinase MASK, STK24_HUMAN, STK24, Mammalian STE20-like protein kinase 3 (MST-3), STE20-like kinase MST3, |
| Phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 48 kDa |
| UniProt: | O00506 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | STK25 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Application Notes: | Modification: Phosphorylated |
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Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174)) Polyclonal Antibody, Unconjugated (orb185140) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174)) Polyclonal Antibody, Unconjugated (orb185140) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174)) Polyclonal Antibody, Unconjugated (orb185140) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174)) Polyclonal Antibody, Unconjugated (orb185140) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174)) Polyclonal Antibody, Unconjugated (orb185140) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: U-87MG (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) (orb185140) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 39 kD. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-MST4+MST3+STK25 (Thr178+Thr190+Thr174) Polyclonal Antibody, Unconjugated (orb185140) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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