Phospho-MAK (Tyr159) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB185427
Article Name: Phospho-MAK (Tyr159) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB185427
Supplier Catalog Number: orb185427
Alternative Catalog Number: BYT-ORB185427-50,BYT-ORB185427-100,BYT-ORB185427-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthesised phosphopeptide derived from human MAK around the phosphorylation site of Tyr159 TD(p-Y)VS
Conjugation: Unconjugated
Alternative Names: MAK (p-Y159), p-MAK, phospho-MAK, RP62, A930010O05Rik, MAK_HUMAN, MAK, Male germ cell-associated kinase, 2.7.11.1, MAK_MOUSE, Protein kinase RCK, Rck, MAK_RAT,
Phospho-MAK (Tyr159) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 71 kDa
UniProt: P20794
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: Mak
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Application Notes: Modification: Phosphorylated
Immunohistochemical staining of rat testis tissue using MAK (phospho-Tyr159) antibody.
Immunohistochemical staining of human prostate tissue using MAK (phospho-Tyr159) antibody.
Protein: k562 (human) lysate at 40 ug, Primary: rabbit Anti-phospho-MAK (Tyr159) (orb185427) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 71 kD, Observed band size: 71 kD.
Sample: Hela (Human) Cell Lysate at 30 ug, Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-phospho-MAK (Tyr159) (orb185427) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 71 kD, Observed band size: 71/66 kD.
Sample: Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-phospho-MAK (Tyr159) (orb185427) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 71 kD, Observed band size: 73 kD.
Tissue/Cell: human prostate tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-MAK (Tyr159) Polyclonal Antibody, Unconjugated (orb185427) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-MAK (Tyr159) Polyclonal Antibody, Unconjugated (orb185427) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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