SRC Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB186006
- Images (7)
| Article Name: | SRC Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB186006 |
| Supplier Catalog Number: | orb186006 |
| Alternative Catalog Number: | BYT-ORB186006-50,BYT-ORB186006-100,BYT-ORB186006-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Src (151-250/536aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | ASV, SRC1, THC6, c-SRC, p60-Src, pp60c-src, SRC_HUMAN, SRC, Proto-oncogene c-Src, pp60c-src (p60-Src), 2.7.10.2, SRC_MOUSE, SRC_RAT, |
| SRC Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 61 kDa |
| UniProt: | P12931 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | SRC |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
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HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (SRC) polyclonal Antibody, Unconjugated (orb186006) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SRC) Polyclonal Antibody, Unconjugated (orb186006) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SRC) Polyclonal Antibody, Unconjugated (orb186006) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (SRC) Polyclonal Antibody, Unconjugated (orb186006) at 1:400 overnight at 4C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SRC) Polyclonal Antibody, Unconjugated (orb186006) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Rat Testis tissue lysates, Primary: Anti-SRC (orb186006) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61 kDa, Observed band size: 61 kDa. |
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IHC-P analysis of Rat testis using SRC Polyclonal Antibody at 1:200 dilution. |
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