anti SIRT4 antibody, anti SIR2L4 antibody, anti sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) antibody, anti sirtuin silent mating type information regulation 2 homolog 4 (S. cerevisiae) antibody, anti MGC130046 antibody, anti MGC130047 antibody, anti MGC57437 antibody, anti SIR2L4 antibody, anti sirtuin 4 antibody, anti sir2-like 4 antibody, anti sirtuin 4 antibody, anti sirtuin type 4 antibody
Goat polyclonal antibody to SIRT4
Clonality:
Polyclonal
Molecular Weight:
35.2
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Sequence:
SRCGELLPLIDPC
Target:
SIRT4
Application Dilute:
ELISA: 1:32000, WB: 1-3 µg/ml, IHC-P: 2-3 µg/ml
Application Notes:
Application Notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Skeletal Muscle shows spotty staining of striated fibres in longitudinal section. Recommended concentration, 2-3ug/ml.WB: Approx. 38kDa band observed in Human Kidney and U937 lysates (calculated MW of 35.2kDa according to NP_036372.1). In transfected HEK293 transiently expressing SIRT4 a band of approx. 38kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 µg/ml
orb18632 (0.75ug/ml) staining of U937 (A), (2ug/ml) K562 (B) and (3ug/ml) U2OS (C), MCF7 (D), and U251 (E) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
orb18632 (7µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
orb18632 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
orb18632 (7µg/ml) staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
orb18632 Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
orb18632 (7µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
orb18632 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
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