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Goat polyclonal antibody to AIRE
Clonality:
Polyclonal
Molecular Weight:
57.7
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Sequence:
QSMARPAAPFPS
Target:
AIRE
Application Dilute:
ELISA: 1:128000, WB: 1-2 µg/ml, IHC-P: 2.5 µg/ml
Application Notes:
Application Notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration, 2.5ug/ml.WB: Appr. 60kDa in transfected HEK293 lysates (calculated MW of 57.7kDa according to NP_000374.1). Recommended concentration: 0.01-0.03 µg/ml.Experiment Notes: ChIP: A customer has reported this antibody was successful in ChIP (no data)
Immunohistochemical staining of Human Thymus using AIRE antibody
Immunohistochemical staining of Human Thymus using AIRE antibody
Western blot analysis of Human Lymph Node lysate using AIRE antibody
Flow cytometric analysis of Jurkat cells using AIRE antibody
Immunofluorescence analysis of HeLa cells using AIRE antibody
Immunofluorescence analysis of U2OS cells using AIRE antibody
WB analysis of Jurkat cell (A), Human Spleen (B) and Human Smooth Muscle (C) lysate protein using AIRE antibody
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