GAPDH (C Terminus) Loading Control Antibody, Unconjugated, Goat, Polyclonal
Biozol Catalog Number:
BYT-ORB18774
Supplier Catalog Number:
orb18774
Alternative Catalog Number:
BYT-ORB18774-100
Manufacturer:
Biorbyt
Host:
Goat
Category:
Antikörper
Application:
ELISA, IF, IHC, WB
Species Reactivity:
Human, Rat
Conjugation:
Unconjugated
Alternative Names:
anti GAPDH antibody, anti glyceraldehyde-3-phosphate dehydrogenase antibody, anti HGNC:4141 antibody, anti G3PD antibody, anti GAPD antibody, anti MGC88685 antibody, anti OTTHUMP00000174431 antibody, anti OTTHUMP00000174432 antibody, anti aging-associated gene 9 protein antibody, anti glyceraldehyde 3-phosphate dehydrogenase antibody
Goat polyclonal antibody to GAPDH
Clonality:
Polyclonal
Molecular Weight:
36.1
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Application Notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 35kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1kDa according to NP_002037). Recommended concentration: 0.01-0.03 µg/ml
0.1 µg/ml staining of Human Liver (A) and (1 ug/ml) Mouse Spleen (B) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
0.1 µg/ml staining of HEK293 (A) and HeLa (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
5 µg/ml staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.
Immunofluorescence analysis of paraformaldehyde fixed A549. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (1:2000 dilution), showing cytoplasmic, membrane and cytoplasmic bodies staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor 488 secondary antibody.
5 µg/ml staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.
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