Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application Dilute:
WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50
Formalin-fixed and paraffin-embedded H.breast tissue reacted with ADH5 Antibody (Center).
Formalin-fixed and paraffin-embedded H.placenta tissue reacted with ADH5 Antibody (Center).
Western blot analysis of ADH5 (arrow) using rabbit polyclonal ADH5 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH5 gene.
Flow cytometric analysis of K562 cells using ADH5 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADH5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.
All lanes: Anti-ADH5 Antibody (Center) at 1:4000 dilution. Lane 1: K562 whole cell lysate. Lane 2: SH-SY5Y whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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