Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application Dilute:
IF - 1:10-50, WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50
Western blot analysis of anti-CDK4 Pab in HL-60 cell lysate. CDK4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of CDK4 (arrow) using rabbit polyclonal CDK4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK4 gene (Lane 2).
CDK4 Antibody (C-term) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human prostate carcinoma with CDK4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated. BC = breast carcinoma, HC = hepatocarcinoma.
Fluorescent image of Hela cell stained with CDK4 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDK4 primary antibody (1: 25, 1 h at 37C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37C).CDK4 immunoreactivity is localized to Cytoplasm significantly.
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