Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application Dilute:
WB - 1:1000
Staining ESRP1 in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
ESRP1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ESRP1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
ESRP1 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35 ug/lane). This demonstrates the ESRP1 antibody detected the ESRP1 protein (arrow).
All lanes: Anti-ESRP1 Antibody (N-term) at 1:1000 dilution. Lane 1: mouse colon tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size: 54kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-ESRP1 Antibody (N-term) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 76 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-ESRP1 Antibody (N-term) at 1:2000 dilution. Lane 1: HCT116 whole cell lysates. Lane 2: MCF-7 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 76 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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