Human A Disintegrin and Metalloproteinase with Thrombospondin 12 (ADAMTS12) ELISA Kit
Biozol Catalog Number:
BYT-ORB1947863
Supplier Catalog Number:
orb1947863
Alternative Catalog Number:
BYT-ORB1947863-48,BYT-ORB1947863-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Species Reactivity:
Human
Alternative Names:
A disintegrin and metalloproteinase with thrombospondin motifs 12,A disintegrin like and metalloprotease with thrombospondin type 1 motif 12,ADAM metallopeptidase with thrombospondin type 1 motif 12,ADAM TS 12
Human A Disintegrin and Metalloproteinase with Thrombospondin 12 (ADAMTS12) ELISA Kit
serum, plasma, Tissue homogenate and Other biological samples
Target:
ADAMTS12
Application Notes:
Application Notes: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADAMTS12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADAMTS12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADAMTS12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Human ADAMTS12. You can calculate the concentration of Human ADAMTS12 in the samples by comparing the OD of the samples to the standard curve
orb1947863
orb1947863
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