Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Application Notes: IHC: Frozen section of Human Hypothalamus shows staining of galaninergic axons in the human periventricular nucleus. Recommended concentration, 0.2-0.5µg/ml.WB: Approx 17kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates (calculated MW of 13.3kDa according to NP_057057.2). Recommended concentration: 1-3 µg/ml
Western blot analysis of Mouse and Rat Brain lysate using Galanin antibody
Western blot analysis of human cerebellum lysate (35µg protein in RIPA buffer) using GAL antibody
orb20373 (1µg/ml) staining of Mouse Brain (A) Small Intestine (B) and Stomach (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
orb20373 (8µg/ml) staining of paraffin embedded Human Small Intestine. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
orb20373 Negative Control showing staining of paraffin embedded Human Small Intestine, with no primary antibody.
Immunohistochemical analysis of formalin-fixed paraffin embedded human hypothalamus tissue using GAL antibody
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