C20orf132 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB2050
Article Name: C20orf132 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB2050
Supplier Catalog Number: orb2050
Alternative Catalog Number: BYT-ORB2050-50,BYT-ORB2050-100,BYT-ORB2050-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human C20orf132 (401-483/483aa)
Conjugation: Unconjugated
Alternative Names: C20orf131, C20orf132, MROH8_HUMAN, MROH8, Maestro heat-like repeat-containing protein family member 8,
C20orf132 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 55 kDa
UniProt: Q9H579
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: MROH8
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Western blot analysis of Mouse brain tissue tissue using C20orf132 antibody.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (C20orf132) Polyclonal Antibody, Unconjugated (orb2050) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (C20orf132) Polyclonal Antibody, Unconjugated (orb2050) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-C20orf132 (orb2050) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55/99 kD, Observed band size: 55/95 kD.
Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-C20orf132 (orb2050) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55kD.
Sample: Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-C20orf132 (orb2050) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55kD.



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