Fas Ligand Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB221406
- Images (7)
| Article Name: | Fas Ligand Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB221406 |
| Supplier Catalog Number: | orb221406 |
| Alternative Catalog Number: | BYT-ORB221406-50,BYT-ORB221406-100,BYT-ORB221406-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Fas Ligand (196-281/281aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | ALPS1B, APT1LG1, APTL, CD178, CD95-L, CD95L, FASL, TNFSF6, TNLG1A, Fas-L, Faslg, gld, TNFL6_HUMAN, Apoptosis antigen ligand (APTL), CD95 ligand (CD95-L), Fas antigen ligand (Fas ligand | FasL), TNFL6_MOUSE, TNFL6_RAT, |
| Fas Ligand Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 38-42 kDa |
| UniProt: | P48023 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | FASLG |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
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Blank control: Mouse Kidney (blue). Primary Antibody: Rabbit Anti-phospho-Fas Ligand antibody (orb221406, Green), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde for 10 min at 37C. Primary antibody (orb221406, 1 µg/1x10 6 cells) were incubated for 30 min at room temperature, followed by 1X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min on ice. Acquisition of 20000 events was performed. |
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Blank control: mouse thymouses (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:100 in 1X PBS containing 0.5% BSA, Primary Antibody, dilution: 1 µl in 100 µl 1X PBS containing 0.5% BSA (green). |
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Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Fas Ligand) Polyclonal Antibody, Unconjugated (orb221406) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Bone (mouse) Lysate at 40 ug, Primary: Anti-Fas ligand (orb221406) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 46 kD. |
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Sample: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-Fas Ligand (orb221406) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 40 kD. |
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Sample: Lane 1: Bone (Mouse) Lysate at 40 ug, Lane 2: Bone (Rat) Lysate at 40 ug, Primary: Anti-Fas Ligand (orb221406) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kD, Observed band size: 40 kD. |
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Tissue/Cell: human rectal carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-FasL Polyclonal Antibody, Unconjugated (orb221406) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei. |
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