E.coli-derived human Parkin recombinant protein (Position: I23-K416). Human Parkin shares 82% and 84% amino acid (aa) sequence identity with mouse and rat Parkin, respectively.
Conjugation:
Unconjugated
Alternative Names:
E3 ubiquitin-protein ligase parkin, Parkin, 6.3.2.-, Parkinson juvenile disease protein 2, Parkinson disease protein 2, PARK2, PRKN
Parkin/PRKN Rabbit Polyclonal Antibody
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation
Form:
Lyophilized
Target:
E3 ubiquitin-protein ligase parkin
Application Dilute:
Immunocytochemistry, 0.5-1µg/ml, Mouse, - Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat
IHC(P) analysis of Mouse Brain Tissue using Anti-Parkin Picoband antibody.
Anti-Parkin Picoband antibody, IHC(P): Human Intestinal Cancer Tissue.
IHC(P) analysis of Rat Brain Tissue using Anti-Parkin Picoband antibody.
Anti-Parkin Picoband antibody, IHC(P): Rat Brain Tissue.
Anti-Parkin Picoband antibody, Western blotting. All lanes: Anti Parkin at 0.5 ug/ml. Lane 1: U87 Whole Cell Lysate at 40 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Predicted bind size: 66 KD. Observed bind size: 66 KD.
IHC analysis of Parkin using anti-Parkin antibody. Parkin was detected in immunocytochemical section of NEURO-2alpha Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 µg/ml rabbit anti-Parkin Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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