A human recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain was used as the immunogen for the Podocalyxin antibody.
Conjugation:
Unconjugated
Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
Clonality:
Monoclonal
Clone Designation:
[3D3]
Buffer:
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Application Dilute:
Flow cytometry: 0.5-1ug/10 6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Notes:
Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Podocalyxin antibody to be titered up or down for optimal performance
Immunfluorescent staining of human HeLa cells with Podocalyxin antibody (clone 3D3, green) and Reddot nuclear stain (red).
IHC staining of FFPE human placenta with Podocalyxin antibody (clone 3D3). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human angiosarcoma with Podocalyxin antibody (clone 3D3). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
FACS staining of human NCCIT cells with Podocalyxin antibody (clone 3D3, red) and an isotype control antibody (green).
Western blot testing of human HeLa cell lysate with Podocalyxin antibody (clone 3D3). Expected molecular weight: 55-165 kDa depending on level of glycosylation.
SDS-PAGE analysis of purified, BSA-free Podocalyxin antibody (clone 3D3) as confirmation of integrity and purity.
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