SDS extract of human myocardium was used as the immunogen for this Muscle Actin HHF35 antibody.
Conjugation:
Unconjugated
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. This mAb recognizes alpha and gamma isotypes of all muscle groups. Nonmuscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of nonmuscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Application Dilute:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Notes:
Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Muscle actin HHF35 antibody to be titered up or down for optimal performance.1. No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
IHC staining of human leiomyosarcoma (10X) with Muscle Actin antibody (HHF35).
IHC staining of human leiomyosarcoma (20X) with Muscle Actin antibody (HHF35).
IHC testing of FFPE rhabdomyosarcoma with Muscle Actin antibody
Western blot testing of 1) human muscle and 2) mouse muscle cell lysate with Muscle Actin antibody (clone HHF35).
Western blot testing of human endometrium muscle lysate with Muscle Actin antibody (clone HHF35).
SDS-PAGE analysis of purified, BSA-free Muscle Actin antibody (clone HHF35) as confirmation of integrity and purity.
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