Cardiac Troponin I/TNIC Mouse Monoclonal Antibody, Clone: [3A9], Unconjugated

Catalog Number: BYT-ORB2563505
Article Name: Cardiac Troponin I/TNIC Mouse Monoclonal Antibody, Clone: [3A9], Unconjugated
Biozol Catalog Number: BYT-ORB2563505
Supplier Catalog Number: orb2563505
Alternative Catalog Number: BYT-ORB2563505-100,BYT-ORB2563505-200,BYT-ORB2563505-50
Manufacturer: Biorbyt
Host: Mouse
Category: Antikörper
Application: IF, IHC-Fr, IHC-P
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant human Cardiac Troponin I (cTnI) (1-210/210aa)
Conjugation: Unconjugated
Alternative Names: CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, Tn1, TnI, TNNI3_HUMAN, TNNI3, Cardiac troponin I, TNNI3_MOUSE, TNNI3_RAT,
Cardiac Troponin I/TNIC Mouse Monoclonal Antibody
Clonality: Monoclonal
Concentration: 1mg/ml
Clone Designation: [3A9]
Molecular Weight: 23 kDa
UniProt: P19429
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: TNNI3
Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Negative Sample: Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Cardiac Troponin I/TNIC) Monoclonal Antibody, Unconjugated (orb2563505) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Negative Sample: Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Cardiac Troponin I/TNIC) Monoclonal Antibody, Unconjugated (orb2563505) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Negative Sample: Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Cardiac Troponin I/TNIC) Monoclonal Antibody, Unconjugated (orb2563505) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Negative Sample: Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Cardiac Troponin I/TNIC) Monoclonal Antibody, Unconjugated (orb2563505) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Negative Sample: Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Cardiac Troponin I/TNIC) Monoclonal Antibody, Unconjugated (orb2563505) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Cardiac Troponin I/TNIC) Monoclonal Antibody, Unconjugated (orb2563505) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Cardiac Troponin I/TNIC) Monoclonal Antibody, Unconjugated (orb2563505) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.




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