Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.6 kDa. The protein migrates as 34-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
SDS-PAGE analysis of Human IL-2 R alpha protein
Human IL-2 R alpha, His Tag (Cat. No. orb257613) captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free with an affinity constant of 29.9 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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