Application Notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 44.3 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
SDS-PAGE analysis of Human IL-18BP protein
Human IL-18BP, Fc Tag (Cat. No. orb257623) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-18, Tag Free with an affinity constant of 1.71 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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