Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 36.6 kDa. The protein migrates as 37-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
SDS-PAGE analysis of Human IL-12B protein
Loaded Human IL-12 R beta 1, Fc Tag (Cat. No. orb570282) on Protein A Biosensor, can bind Human IL-12B, His Tag (Cat. No. orb257709) with an affinity constant of 3.78 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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