Human milk fat globule membranes were used as the immunogen for this EMA antibody.
Conjugation:
Unconjugated
This antibody recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of MUC1 (Mucin-1) or EMA (epithelial membrane antigen). The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. MUC1 / Mucin-1 may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling through phosphorylations and protein-protein interactions. In immunohistochemical assays, the MUC1 / EMA antibody superbly stains routine formalin/paraffin carcinoma tissues. MUC1 antibody is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Clonality:
Monoclonal
Clone Designation:
[MUC1/845]
Buffer:
1 mg/ml in 1X PBS, rAlbumin free, sodium azide free
Application Dilute:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EMA antibody to be titered up or down for optimal performance.1. HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 10-20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EMA antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human colon carcinoma stained with EMA antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with EMA antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC1 / EMA antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody (MUC1/845).
SDS-PAGE Analysis of Purified, BSA-Free EMA Antibody (clone MUC1/845). Confirmation of Integrity and Purity of the Antibody.
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