Chromogranin A Antibody, Clone: [CHGA/798], Unconjugated, Mouse, Monoclonal
Biozol Catalog Number:
BYT-ORB2638303
Supplier Catalog Number:
orb2638303
Alternative Catalog Number:
BYT-ORB2638303-100
Manufacturer:
Biorbyt
Host:
Mouse
Category:
Antikörper
Application:
IHC-P
Species Reactivity:
Human, Rat
Immunogen:
Recombinant human protein was used as the immunogen for the Chromogranin A antibody.
Conjugation:
Unconjugated
Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
1 mg/ml in 1X PBS, rAlbumin free, sodium azide free
Application Dilute:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
Application Notes: Optimal dilution of the Chromogranin A antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
IHC: Formalin-fixed, paraffin-embedded human adrenal gland stained with Chromogranin A antibody (CHGA/798)
IHC: Formalin-paraffin human pancreas stained with Chromogranin A antibody (CHGA/798)
IHC: Formalin-paraffin rat pancreas stained with Chromogranin A antibody (CHGA/798)
IHC: Formalin-fixed, paraffin-embedded human parathyroid stained with Chromogranin A antibody (CHGA/798)
IHC: Formalin-paraffin human SC lung carcinoma stained with Chromogranin A antibody (CHGA/798)
Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Chromogranin A antibody (clone CHGA/798). These results demonstrate the foremost specificity of the CHGA/798 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Chromogranin A antibody (clone CHGA/798) as confirmation of integrity and purity.
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