Recombinant full-length human protein was used as the immunogen for the anti-Vimentin antibody.
Conjugation:
Unconjugated
This mAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
1 mg/ml in 1X PBS, rAlbumin free, sodium azide free
Application Dilute:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
Application Notes: Optimal dilution of the anti-Vimentin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
IHC: Formalin-fixed, paraffin-embedded human melanoma stained with anti-Vimentin antibody (clone VM1170).
IHC: Formalin-fixed, paraffin-embedded human angiosarcoma stained with anti-Vimentin antibody (clone VM1170).
IHC: Formalin-fixed, paraffin-embedded human Ewings sarcoma stained with anti-Vimentin antibody (clone VM1170).
IHC: Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with anti-Vimentin antibody (clone VM1170).
IHC: Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with anti-Vimentin antibody (clone VM1170).
IHC: Formalin-fixed, paraffin-embedded human uterus stained with anti-Vimentin antibody (clone VM1170).
Western blot testing of human HepG2 cell lysate with anti-Vimentin antibody. Predicted molecular weight ~58 kDa.
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