A partial human recombinant protein was used as the immunogen for the Calnexin antibody.
Conjugation:
Unconjugated
It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
1 mg/ml in 1X PBS, rAlbumin free, sodium azide free
Application Dilute:
ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml
Application Notes:
Application Notes: Titering of the Calnexin antibody may be required for optimal performance
IHC testing of FFPE human small intestine carcinoma with Calnexin antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0 for 10-20 min.
IHC testing of FFPE human renal cell carcinoma with Calnexin antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0 for 10-20 min.
IHC testing of FFPE human renal cell carcinoma with Calnexin antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0 for 10-20 min.
Western blot testing of human kidney lysate with Calnexin antibody (clone CANX/1541). Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.
Western blot testing of human 1) HeLa and 2) MCF7 cell lysate with Calnexin antibody (clone CANX/1541). Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.
Immunofluorescent staining of human HeLa cells with Calnexin antibody (green) (clone CANX/1541). RedDot used as nuclear counterstain (red).
SDS-PAGE analysis of purified, BSA-free Calnexin antibody (clone CANX/1541) as confirmation of integrity and purity.
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