Human tonsil B cells were used as the immunogen for this CD20 antibody.
Conjugation:
Unconjugated
This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V, Code CD20.12). The antibody recognizes the extracellular domain of the protein. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of Bcells and its expression is restricted to normal and neoplastic Bcells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of Bcell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting Bcells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in Bcell activation and cell cycle progression.
1 mg/ml in 1X PBS, rAlbumin free, sodium azide free
Application Dilute:
Flow cytometry: 1-2ug/10 6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD20 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
IHC staining of FFPE human tonsil tissue with CD20 antibody (clone L26). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human lymphoma with CD20 antibody (clone L26). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Immunofluorescence staining of human Raji cells with CD20 antibody (green, clone L26) and Reddot nuclear stain (red).
Flow cytometry testing of human Raji cells with CD20 antibody (clone L26), Red=isotype control, Blue= CD20 antibody.
Western blot testing of human Raji lysate with CD20 antibody (clone L26). Predicted molecular weight ~33 kDa.
SDS-PAGE analysis of purified, BSA-free CD20 antibody (clone L26) as confirmation of integrity and purity.
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