Partially purified HSP27 protein from the human breast cancer cell line MCF-7 was used as the immunogen for this HSP27 antibody.
Conjugation:
Unconjugated
It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
1 mg/ml in 1X PBS, rAlbumin free, sodium azide free
Application Dilute:
Flow cytometry: 1-2ug/10 6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Notes:
Application Notes: The optimal dilution of the HSP27 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (clone SPM252).
IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (clone SPM252).
Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP27 antibody (clone SPM252, green) and Reddot nuclear stain (red).
Flow cytometry testing of PFA-fixed human MCF7 cells with HSP27 antibody (clone SPM252), Red=isotype control, Blue= HSP27 antibody.
Western blot testing of human HeLa cell lysate with HSP27 antibody (clone SPM252). Expected molecular weight: 24-29 kDa.
SDS-PAGE analysis of purified, BSA-free HSP27 antibody (clone SPM252) as confirmation of integrity and purity.
* VAT and and shipping costs not included. Errors and price changes excepted
