Elastin Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB2881
- Images (6)
| Article Name: | Elastin Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB2881 |
| Supplier Catalog Number: | orb2881 |
| Alternative Catalog Number: | BYT-ORB2881-50,BYT-ORB2881-100,BYT-ORB2881-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Elastin (101-200/786aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | ADCL1, SVAS, WBS, WS, E030024M20Rik, ELN_HUMAN, ELN, Tropoelastin, ELN_MOUSE, |
| Elastin Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 68 kDa |
| UniProt: | P15502 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | ELN |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Elastin) Polyclonal Antibody, Unconjugated (orb2881) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: Lane 1: Lung (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Uterus (Rat) Lysate at 40 ug, Lane 4: Liver (Rat) Lysate at 40 ug, Primary: Anti-Elastin (orb2881) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50-80 kD, Observed band size: 75 kD. |
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Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-Elastin (orb2881) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 80 kD, Observed band size: 80 kD. |
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Sample: Uterus (Mouse) Lysate at 40 ug, Primary: Anti-Elastin (orb2881) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 80 kD, Observed band size: 80 kD. |
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Tissue/Cell: human cervical cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Elastin Polyclonal Antibody, Unconjugated (orb2881) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: mouse stomach wall, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Elastin Polyclonal Antibody, Unconjugated (orb2881) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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