Activated Notch1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB312158
Article Name: Activated Notch1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB312158
Supplier Catalog Number: orb312158
Alternative Catalog Number: BYT-ORB312158-50,BYT-ORB312158-100,BYT-ORB312158-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Mouse
Immunogen: KLH conjugated synthetic peptide derived from human N-terminal sequence of the cleaved Notch1 intracellular domain (1754-1800/2555aa)
Conjugation: Unconjugated
Alternative Names: AOS5, AOVD1, TAN1, hN1, 9930111A19Rik, Mis6, N1, lin-12, NOTCH, NOTC1_HUMAN, NOTCH1, Notch 1, Translocation-associated notch protein TAN-1, NOTC1_MOUSE, Motch A, mT14, p300, Motch, NOTC1_RAT,
Activated Notch1 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 271 kDa
UniProt: P46531
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: NOTCH1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of Mouse brain using Activated Notch1 antibody.
Western blot analysis of Mouse spleen Lysate using Activated Notch1 antibody.
Immunohistochemical staining of Mouse lung using Activated Notch1 antibody.
Blank control (blue line): Hela (fixed with 70% ethanol overnight at 4C and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Activated Notch1 antibody (orb312158), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Activated Notch1) Polyclonal Antibody, Unconjugated (orb312158) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Activated Notch1) Polyclonal Antibody, Unconjugated (orb312158) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Sample: spleen (mouse) Lysate at 40 ug, Primary: Anti-Activated Notch1 (orb312158) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 86kD, Observed band size: 107kD.



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