CD4 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB312176
Article Name: CD4 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB312176
Supplier Catalog Number: orb312176
Alternative Catalog Number: BYT-ORB312176-50,BYT-ORB312176-100,BYT-ORB312176-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from the middle of mouse CD4 (231-330/457aa)
Conjugation: Unconjugated
Alternative Names: CD4mut, IMD79, Leu-3, OKT4D, T4, CD4_HUMAN, CD4, T-cell surface antigen T4/Leu-3, CD4_MOUSE, T-cell differentiation antigen L3T4, L3T4, Ly-4, CD4_RABIT,
CD4 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 55 kDa
UniProt: P06332
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: CD4
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Flow cytometric analysis of mouse splenocytes Cell using CD4 antibody.
Western blot analysis of 293T cell lysate using CD4 antibody.
Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (orb312176) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Lane 1: Human HL-60 cell lysates, Lane 2: Human MOLT4 cell lysates, Lane 3: Human Jurkat cell lysates, Lane 4: Human Raji cell lysates, Primary: Anti-CD4 (orb312176) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kDa >, Observed band size: 60 kDa.
Sample: U937 (Human) Cell Lysate at 30 ug, Primary: Anti-CD4 (orb312176) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 55 kD.
Tissue/Cell: rat lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CD4 (mouse, rat) Polyclonal Antibody, Unconjugated (orb312176) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.



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