Phospho-CDKN1B (Ser10) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB312189
Article Name: Phospho-CDKN1B (Ser10) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB312189
Supplier Catalog Number: orb312189
Alternative Catalog Number: BYT-ORB312189-50,BYT-ORB312189-100,BYT-ORB312189-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthesised phosphopeptide derived from human CDKN1B around the phosphorylation site of Ser10 NG(p-S)PS
Conjugation: Unconjugated
Alternative Names: CDKN1B (p-S10), p-CDKN1B, phospho-CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27KIP1, p27, Cdki1b, CDN1B_HUMAN, CDKN1B, Cyclin-dependent kinase inhibitor p27, CDN1B_MOUSE,
Phospho-CDKN1B (Ser10) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 22 kDa
UniProt: P46527
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: CDKN1B
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
Application Notes: Modification: Phosphorylated
Flow cytometric analysis of MCF7 cell using CDKN1B (phospho-Ser10) antibody.
Blank control (blue line): MCF7 (fixed with 70% ethanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 30 min on ice), Primary Antibody (green line): Rabbit Anti-phospho-CDKN1B (Ser10) antibody (orb312189), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-CDKN1B (Ser10)) Polyclonal Antibody, Unconjugated (orb312189) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-CDKN1B (Ser10)) Polyclonal Antibody, Unconjugated (orb312189) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lymphocyte (Rat) Lysate at 40 ug, Primary: Anti-phospho-CDKN1B (Ser10) (orb312189) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22kD.
Sample: MOLT-4 Cell (Human) Lysate at 30 ug, Primary: Anti-phospho-CDKN1B (Ser10) (orb312189) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22kD.
Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-phospho-CDKN1B (Ser10) (orb312189) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22kD.



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