EpCAM Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB312226
- Images (6)
| Article Name: | EpCAM Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB312226 |
| Supplier Catalog Number: | orb312226 |
| Alternative Catalog Number: | BYT-ORB312226-50,BYT-ORB312226-100,BYT-ORB312226-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human, Mouse |
| Immunogen: | KLH conjugated synthetic peptide derived from human EpCAM (53-150/314aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | Ber-Ep4, BerEp4, DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, LYNCH8, M4S1, MIC18, MK-1, MOC-31, TACSTD1, TROP1, CD326, EGP, Ep-CAM, EpCAM1, GA733-2, Ly74, Tacsd1, gp40, EPCAM_HUMAN, EPCAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein (EGP), Epithelial glycoprotein 314 (EGP314 | hEGP314), KS 1/4 antigen, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, M1S2, EPCAM_MOUSE, Epithelial glycoprotein 314 (EGP314 | mEGP314), Protein 289A, EPCAM_RAT, Epithelial glycoprotein 314 (EGP314), Protein D5.7A, |
| EpCAM Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 40 kDa |
| UniProt: | P16422 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | EPCAM |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Immunohistochemical staining of Rabbit bladder tissue using EpCAM antibody. |
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Immunohistochemical staining of mouse embryo tissue using EpCAM antibody. |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb312226) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb312226) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-EpCAM Polyclonal Antibody, Unconjugated (orb312226) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-EpCAM Polyclonal Antibody, Unconjugated (orb312226) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei. |
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