Immunohistochemical staining of human breast cancer tissue using HSF1 (phospho-Ser326) antibody.
Immunohistochemical staining of human breast cancer tissue using HSF1 (phospho-Ser326) antibody.
Blank control: A549. Primary Antibody (green line): Rabbit Anti-phospho-HSF1 (Ser326) antibody, dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (p-HSF1) Polyclonal Antibody, Unconjugated (orb312275) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human neurinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-HSF1 (Ser326)) Polyclonal Antibody, Unconjugated (orb312275) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Tissue/Cell: human liver carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-HSF1 (Ser326) polyclonal Antibody, Unconjugated (orb312275) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
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