Phospho-NFKB1 (Ser373) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB312392
Article Name: Phospho-NFKB1 (Ser373) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB312392
Supplier Catalog Number: orb312392
Alternative Catalog Number: BYT-ORB312392-50,BYT-ORB312392-100,BYT-ORB312392-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human NF KappaB p105 around the phosphorylation site of Ser373 SD(p-S)FG
Conjugation: Unconjugated
Alternative Names: NFKB1 | NFkB p105 (p-S373), p-NFkB p105, phospho-NFkB p105, CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB, NF-kappaB1, p105, p50, p50/p105, NFKB1_HUMAN, NFKB1, DNA-binding factor KBF1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, NFKB1_MOUSE, NF-kappa-B1 p84/NF-kappa-B1 p98, NFKB1_RAT,
Phospho-NFKB1 (Ser373) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 48/105 kDa
UniProt: P19838
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: NFKB1
Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
Application Notes: Modification: Phosphorylated
Flow cytometric analysis of A549 cells using NFKB1(phospho-Ser373) antibody.
Blank control (blue line): A549 (fixed with 70% ethanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-NFKB1 (Ser373) antibody (orb312392), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-NFKB1 (Ser373)) Polyclonal Antibody, Unconjugated (orb312392) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-NFKB1 (Ser373)) Polyclonal Antibody, Unconjugated (orb312392) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-NFKB1 (Ser373)) Polyclonal Antibody, Unconjugated (orb312392) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-NFKB1 (Ser373)) Polyclonal Antibody, Unconjugated (orb312392) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.



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